How to Cite
Publication Dates
Received
Revised
Accepted
Published Online First
Abstract
This study aimed to utilise response surface methodology (RSM) to determine the optimal conditions for extracting urease from a plant source and evaluate its biochemical properties. Chickpea seeds were selected as the source of urease. Results showed that chickpea seeds had a high enzyme activity (3.99 U/mg protein) under the best extraction conditions of pH 7.99, tris base buffer concentration of 0.2, time of 60 minutes and ratio of 1:13 (g: mL). When a second-order polynomial model was used, the predicted correlation coefficient (pred. R2) was 0.8477, the adjusted correlation coefficient (adj. R2) was 0.9333 and the correlation values (R2) were 0.9665, indicating that the predicted models and experimental values agreed very well. The method has the potential to enhance the cost-effectiveness of enzyme production through large-scale manufacturing and offers an economical alternative to other techniques.
References
A.M. Barrios, and S. J Lippard, "Interaction of urea with a hydroxide bridged dinuclear nickel centre: an alternative model for the mechanism of urease," J. Am. Chem.Soc, pp. 122: 9172-9177, 2000.
Dalal Subree Bedan. "Extraction, Precipitation and Characterization of Urease from Vicia Faba L ".Al-Mustansiriyah Journal of Science ISSN: ISSN:2521-3520, 2020.
J. Liu, Y. Xu, Y. Nie and G.A. Zhao, "Optimization production of acid urease by Enterobacter sp. in an approach to reduce urea in Chinese rice wine," Bioprocess Biosyst. Eng., vol. 35, pp. 651-658, 2012.
A.B. Becker-Ritt, A.H. Martinelli, S. Mitidieri, V. Feder, G.E. Wasserman, M.H. Vainstein, J.T. Oliveira, L.M. Fiuza, G. Pasqualli and C.R. Carlini., "Antifungal activity of plant and bacterial ureases.," Toxicon, vol. 50, pp. 971-983, 2007.
Krishna, B.L., Singh, A.N., Patra, S., and Dubey, V.K Purification, characterization and immobilization of urease from Momordica charantia seeds. Process Biochemistry, 46 (7), 1486–1491, 2011.
Neda 2021. The Properties and Longevity of Crude Urease Extract for Biocementati.
Qin Y, Cabral JMS: Review properties and applications of urease. Biocatalys and Biotransform 2002, 20(1):1–14.
El-Hefnawy, M.E., M. Sakran, A. Ismail, and E.F. Aboelfetoh. Extraction, purification, kinetic and thermodynamic properties of urease from germinating Pisum Sativum L. seeds. BMC Biochemistry Journal. 15:15, 2014.
B. Hu, K. Zhou, Y. Liu, A. Liu and Q. Zhang, Industrial Crops and Products, 115, 290(2018), DOI:10.1016/j.indcrop.2018.02.034.
Babazadeh, N. S., Salehabadi, H., Zeidabadi, F., Souri, E. and M. Amanlou.. Study of urease inhibitory activity by medicinal plant extracts based on new catalyst for Berthelot reaction and Taguchi experimental design. Journal of the Iranian Chemical Society 15(Suppl):1-8. 2017.
Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Analytical Biochemistry Journal. 72: 248-254. 1976.
Lyer, P. K., Priya, V. V. and R. Gayathri. Assessment of urease activity in Pisum sativum seeds. Drug Invention Today.10: (9), 2018.
Yoosefian, M., S. Ahmadzadeh, M. Aghasi, and M. Dolatabadi. Optimization of electrocoagulation process for efficient removal of ciprofloxacin antibiotic using iron electrode; kinetic and isotherm studies of adsorption. J. Mol. Liq. 225: 544– 553, 2017.
Anderson, M.J., Whitcomb, P.J. Response Surface Methods (RSM) For Peak Process Performance At The Most Robust Operating Conditions, (2015).
Bhattacharya, Scentral Composite Design For Response Surface Methodology And Its Application In Pharmacy. Response Surface,(2021).
Kenton, W. Analysis Of Variance (ANOVA) Explanation, Formula, And Applications,(2022).
Hussein, S. I., Khalaf, A. F., Sameh M. A. and M. T. Salah. Determination of the optimum conditions for urease inhibition extracted from some local plants. Iraqi Journal of Agricultural Sciences. 52(4):802-809, 2021.
Nilsang, S., Lertsiri, S., Suphantharika, M., & Assavanig, AOptimization of enzymatic hydrolysis of fish soluble concentrate by commercial proteases. Journal of Food Engineering, 70(4), 571-578. (2005).
Mohammed, S. J., Mohammed-Ridha, M. JOptimization of levofloxacin removal from aqueous solution using electrocoagulation process by response surface methodology. Iraqi J. Agric. Sci., 52(1), 204-217,(2021).
Ridha, M.J.M., Hussein, S.I., Alismaeel, Z.T., Atiya, M.A., Aziz, G.M. Biodegradation of reactive dyes by some bacteria using response surface methodology as an optimization technique. Alexandria Eng. J. 59(5), 3551–3563., 2020.
Guisan, J.M. 2014. Immobilization of enzymes and cells.2nd ed. Madrid, Spain.
Ghazi, M. A., S. I. Hussein, S. D. Abbass, A. L. Ibrahim, and D. K. Abbas. 2021. Degradation of reactive dyes using immobilized peroxidase purified from Nigella sativa. Iraqi Journal of Agricultural Sciences. 52(6):1365-1374.
Mohanad J. M-Ridha, Amaal A. H, Sahar I. H. DETERMINATION OF THE OPTIMUM CONDITIONS FOR UREASE EXTRACTED FROM SOME LOCAL PLANTS, Iraqi Journal of Agricultural Sciences,2023.
Mahmood, B., F. Extraction, Purification and Characterization of Laccase from Locally Isolate of Cladophora sp. and using in Decolarization of Some Industrial Dyes. Thesis. University of Anbar, College of Education for Pure Science, Department. 2018.
Sieprawska, A.K., K. Rafinska, J.W. Skierska, and B. Buszewski. The Influence of Plant Material Enzymatic Hydrolysis and Extraction Conditions on the Polyphenolic Profiles and Antioxidant Activity of Extracts: A Green and E_cient Approach, 2020.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2023 Al-Khwarizmi Engineering Journal