كيفية الاقتباس
تواريخ المنشور
الإستلام
النسخة النهائية
الموافقة
النشر الالكتروني
الملخص
الخلاصة
هدفت هذه الدراسة الى التحقق من منهجيه سطح الاستجابة (RSM)لتقيم الظروف المثلى لاستخلاص انزيم اليوريز من مصدر نباتي وتقيم خواصه الكيموحيوية حيث تم اختيار بذور الحمص كمصدر لانزيم اليوريز. اظهرت النتائج ان بذور الحمص امتلك اعلى فعالية انزيمه 3.99 وحدة /ملغم بروتين عند افضل الظروف استخلاص على التوالي ( برقم هايدروجيني 7.99,تركيز 0.2 مولاري , زمن 60 دقيقه, نسبه 1:13(وزن: حجم).وبين ان البيانات التجريبه هي افضل تركيبها للنموذج متعدد الحدود من المرتبة الثانية مع عامل الارتباط المتوقع (pred. R2 )هو 0.8477,معامل الارتباط المعدل (Adj. R2 )هو 0.9333وقيم الارتباط 0.9665 R2 وهذا يدل على ان النماذج المتوقعة والقيم التجريبية هي في اتفاق جيد.وهذه الطريقة واعده ويمكن الاستفادة منها في المستقبل ,إضافة إلى ذلك ، تحسين فعالية تكلفة هذه الإنزيمات من خلال الإنتاج الضخم ، مما يجعلها خيارًا أكثر جدوى من الناحية الاقتصادية مقارنة بالطرق الأخرى.
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