Abstract
This study was conducted to determine the effects of concentration of hydrochloric acids, temperature, and time on the hydrolysis of soya proteins (defatted soya flour) by determining the value of total protein nitrogen concentration, and amino nitrogen concentration of protein, peptides, and amino acids, and then calculated the hydrolysis rate of proteins.
The variables of the conditions of hydrolysis process was achieved in this study with the following range value of tests parameter:
- Concentration of HCl solution ranged between 1-7 N,
- Hydrolysis temperature ranged between 35-95 °C, and
- The time of hydrolysis period ranged between 0.5-24 hr.
Experiments were designed according to the central composite rotatable design.
The practical study has shown the possibility of decreasing the negative effect of the acid on the biological characteristics of the protein; then affecting the possibility of using the product for biological purposes (for medical and microbiological laboratories) by:
- Decreasing the acid concentration used in the process of hydrolysis, firstly, and
- Decreasing the temperature of the hydrolysis process, secondly, and then
- Increasing the period of the time of hydrolysis process, thirdly.
References
[2] Weininger, S. J., Stermitz, “Organic Chemistry”, International Edition, (1984).
[3] Friedman, M., and R. Liardon, J. Agric. Food Chem., 33, 666, (1985).
[4] Haurowitz, F., “Biochemistry”, An Introductory Textbook, John Wiley & Sons, Inc., New York, (1955).
[5] Hendrickson, J. B., D. J. Cram, and G. S. Hammond, “Organic Chemistry”, 3rd ed., International Student Edition, McGraw-Hill Kogakusha, Ltd., (1970).
[6] “The Oxoid Manual”, United Kingdom, (1988).
[7] Brewster, R. Q, and W. E. Mcewen, “Organic Chemistry”, 3rd ed., (1958).
[8] “Difco Manual of Dehydrated Culture Media and Reagents for Microbiological and Clinical Laboratory Procedures”, 9th ed., Detroit l, Michigan, U.S.A., (1964).
[9] Minor, L. J., J. Fd. Ind., 47, 758, (1945).
[10] Hirs, C. H. W., W. H. Stein, and S. Moore, J. Biol. Chem., 211, 941, (1954).
[11] Pham, C. B., and R. R. D. Rosario, J. Fd. Technol., 18, 21, (1983).
[12] Pham, C. B., and R. R. D. Rosario, J. Fd. Technol., 18, 163, (1983).
[13] Dzanic, H., I. Mujic, and V. S. Hack, J Agric. Food Chem., 33, 683, (1985).
[14] AL-Aaraji ,S. B., M.Sc. Thesis, University of Baghdad, (1994).
[15] Davies, J. S., “Amino Acids, Peptides and Proteins”, A Review of the Literature Published during 1995, The Royal Society of Chemistry, (1995).
[16] Cochran, W. G., and G. M. Cox, “Experimental Design”, John Wiley and Sons, Inc., New York, (1957).
[17] Montgomery, D. C., “Design and Analysis of Experiments”, John Wily and Sons, New York, (1976).
[18] Perry’s, R. H., and C. H. Chilton, “Chemical Engineers Handbook”, McGraw-Hill, 5th ed., (1973).
[19] Apostolatos, G., J. Fd. Technol., 19, 639, (1984).
[20] Metwalli, N. H., S. I. Shalabi, A. S. Zahran, and O.EL - Demerdash , J. Fd. Technol., 17, 71, (1982).
[21] Whitaler, J. R., and P. E. Granum, Anal. Biochem., 109, 156, (1980).
[22] “AOAC-Official Methods of Analysis”, 12th ed., Association of Official Agricultural Chemists, Washington D. C. (1975).
[23] Lees, R., “Food Analysis: Analytical and Quality Control Methods for the Food Manufacturer, and Buyer”, 3rd ed., Leonard Hill Book, (1975).
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