Bioremediation of Soil Contaminated with Diesel using Biopile system

  • Noor Mohsen Jabbar Department of Biochemical Engineering/ Al-Khwarizmi College of Engineering / University of Baghdad
  • Estabriq Hasan Kadhim Treatment and Disposal of Hazardous Waste Directorate/ Ministry. of Higher Education. and Scientific. Research
  • Alaa Kareem Mohammed Department of Biochemical Engineering/ Al-Khwarizmi College of Engineering / University of Baghdad
Keywords: Bioremediation, bio piles, Diesel, Soil pollution.


This study was focused on biotreatment of soil which polluted by petroleum compounds (Diesel) which caused serious environmental problems. One of the most effective and promising ways to treat diesel-contaminated soil is bioremediation. It is a choice that offers the potential to destroy harmful pollutants using biological activity. The capability of mixed bacterial culture was examined to remediate the diesel-contaminated soil in bio piling system. For fast ex-situ treatment of diesel-contaminated soils, the bio pile system was selected. Two pilot scale bio piles (25 kg soil each) were constructed containing soils contaminated with approximately 2140 mg/kg total petroleum hydrocarbons (TPHs). The amended soil: (contaminated soil with the addition of nutrients and bacterial inoculum), where the soil was mixed with 1.5% of sawdust, then supplied with the necessary nutrients and watered daily to provide conditions promoting microorganism growth. Unamended soil was prepared as a control (contaminated soil without addition).  Both systems were equipped with oxygen to provide aerobic conditions, incubated at atmospheric temperature and weekly sampling within 35 days. Overall 75% of the total petroleum hydrocarbons were removed from the amended soil and 38 % of the control soil at the end of study period.

The study concluded that ex-situ experiment (Bio pile) is a preferable, economical, and environmentally friendly procedure, thus representing a good option for the treatment of soil contaminated with diesel.


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Atanaskovic I., Petrovic J.J., Biocanin M., Karlicic V., Raicevic V., and Lalevic B. (2016). “Stimulation of Diesel Degradation and Biosurfactant Production by Aminoglycosides in a Novel Oil-Degrading Bacterium Pseudomonas Luteola PRO23”. Hemijska Industrija ,70 (2), pp.143–50.

Sharma S. (2012). “Bioremediation: Features, Strategies and Applications”. Asian Journal of Pharmacy and Life Science, 2 (2), pp.202–213.

Riffaldi R., Levi-Minzi R., Cardelli R., Palumbo S., and Saviozzi A. (2006). “Soil Biological Activities in Monitoring the Bioremediation of Diesel Oil-Contaminated Soil”. Water, Air, and Soil Pollution, 170 (1–4), pp. 3–15.

Menezes B. F., Camargo F., Okeke B, and Frankenberger W. (2003). “Bioremediation of Soil Contaminated by Diesel Oil”. Brazilian Journal of Microbiology, 34 (1), pp.65–68.

Ahmed A.W., Alzubaidi F. S., and Hamza S. J. (2014). “Biodegradation of Crude Oil in Contaminated Water by Local Isolates of Enterobacter Cloacae”. Iraqi Journal of Science , 55(3), pp.1025- 33.

Poyntner C. (2014). “Bioremediation of Waste Gas and Soil by Black Extremotolerant Fungi”.M.Sc., Thesis. Department of Biotechnology . University of Natural Resources and Life Sciences. pp.111.

Delille D., Duvala A., and Pelletier E. (2008). “Highly Efficient Pilot Biopiles for on-Site Fertilization Treatment of Diesel Oil- Contaminated Sub-Antarctic Soil”. Cold Regions Science and Technology, 54(1), pp.7-18.

Kuppusamy S., Palanisami T., Megharaj M., Venkateswarlu K., and Naidu R. (2016). “Ex-Situ Remediation Technologies for Environmental Pollutants : A Critical Perspective”. Switzerland: Springer International Publishing. pp.191.

Motsara M. R., and Roy R. N. (2008). “Guide to Laboratory Establishment for Plant Nutrient Analysis ”. Italy: FAO. pp. 119.

Mohee R., and Mudhoo A. (2012). “Bioremediation and Sustainability: Research and Applications”. Hoboken: John Wiley & Sons.pp.431.

Padayachee D., and Lin J. (2011). “The Effect of Fertilizer Amendment on Diesel Biodegradation in Contaminated Soils”. African Journal of Microbiology Research, 5 (14), pp. 1729–1739.

Karamalidis A. K., Evangelou A. C., Karabika E., Koukkou A.I., Drainas C., and Voudrias E.V. (2010). “Bioresource Technology Laboratory Scale Bioremediation of Petroleum-Contaminated Soil by Indigenous Microorganisms and Added Pseudomonas Aeruginosa Strain Spet”. Bioresource Technology, 101 (16), pp. 45–52.

Cho D., Lee I., Oh H., and Kim H. (2007). “Monitoring of Microbial Diversity and Activity During Bioremediation of Crude Oil-Contaminated Soil with Different Treatments”. J. Microbiol Biotechnol. 17, pp. 67–73.

Kaszycki P., Pawliki M., Petryszak P., and Koloczek H. (2010). “ Aerobic proccess for in situ bioremediation of petroleum -drived contamination of soil: Afield study based on laborotory microcosm tests”. Ecological chemisty and engineering, 17 (4), pp.405-14.

Nwadinigwe A. O., and Onyeidu E. G. (2012). “Bioremediation of Crude Oil-Polluted Soil Using Bacteria and Poultry Manure Monitored through Soybean Productivity”. Polish Journal of Environmental Studies, 21 (1), pp. 171–176.

Abd Burghal A. (2015). “Biodegradation and bioremediation of petroleum hydrocarbons by Streptomyces and Nocardiopsis strains isolated from oil contaminated soil of Basrah Governorate-Iraq. Ph.D.,Thesis. College of Science.University of Basrah, pp.142.

Gogoi B., Dutta N., Goswami P., Mohan T. (2003). “A Case Study of Bioremediation of Petroleum-Hydrocarbon Contaminated Soil at a crude oil spill site”. Advances in Environmental Research, 7(4), pp. 767-782.

Snelgrove J. (2010). “Biopile Bioremediation of Petroleum Hydrocarbon Contaminated Soils from a Sub-Arctic Site”. M.Sc., Thesis. Department of Civil Engineering and Applied Mechanics. McGill University. pp. 81.

Umar A., Ujah U. J., Hauwa B., Sumayya B.I., Shuaibu M., Yakubu M.S., and Mina F. U. (2013). “Biodegradation of Waste Lubricating Oil by Bacteria Isolated from the Soil”. Journal Of Environmental Science, Toxicology And Food Technology, 3 (6), pp. 32–37.

Obiakalaije UM, Makinde O.A., Amakoromo E.R. (2015). “Bioremediation of Crude Oil Polluted Soil Using Animal Waste”. International Journal of Environmental Bioremediation & Biodegradation, 3 (3), pp. 79–85.

Shabira G., Afzal M., Anwar F., Tahseen R., Khalid Z. (2008). “Biodegradation of Kerosene in Soil by a Mixed Bacterial Culture under Different Nutrient Conditions”. International Biodeterioration & Biodegradation, 14, pp.35–47

Rizzo A. C., Santos R., Santos R. L., Soriano A. U., Cunha C. D., Rosado A. S., Sobral L., and Leite S. (2010). “Petroleum-Contaminated Soil Remediation in a New Solid Phase Bioreactor”. Journal of Chemical Technology and Biotechnology, 85 (9), pp.1260–67.

Cerqueira V. S., Hollenbach E. B., Maboni F., Vainstein M. H., Camargo F., Peralba M., and Bento F.(2011). “Biodegradation Potential of Oily Sludge by Pure and Mixed Bacterial Cultures”. Bioresource Technology, 102 (23), pp.11003–10.

Shah D., Hasan S. A., and Alamgir A. (2015). “Biodegradation of Crude Petroleum Oil by Indigenous and Isolated Bacterial Strains”. American-Eurasian J. Agric. & Environ. Sci., 15 (9), pp.1827–1834.

Gomes B., Soriano A. U. , Miranda R., Sousa M., and Pereira N. (2009). “Biodegradation of Stored Jet Fuel by a Nocardia Sp. Isolated from Contaminated Soil”. Brazilian Archives of Biology and Technology, 52 (5), pp. 79–84.

Riskuwa-shehu M. L., and Ijah U.J.( 2016). “Enhanced Removal of Crude Oil in Soil by Mixed Culture of Bacillus Megaterium UL05 and Pseudomonas Aeruginosa UL07”. International Journal of Environmental Bioremediation & Biodegradation, 4 (1), pp. 8–12.

Bento F. M., Camargo F. A., Okeke B. C., and Frankenberger W. T. (2005). “Comparative Bioremediation of Soils Contaminated with Diesel Oil by Natural Attenuation, Biostimulation and Bioaugmentation”. Bioresource Technology, 96 (9), pp.1049–55.

Adesodun J. K., and Mbagwu J. S. (2008). “Biodegradation of Waste-Lubricating Petroleum Oil in a Tropical Alfisol as Mediated by Animal Droppings”. Bioresource Technology , 99 , pp. 59–65.

How to Cite
Jabbar, N., Kadhim, E., & Mohammed, A. (2018). Bioremediation of Soil Contaminated with Diesel using Biopile system. Al-Khwarizmi Engineering Journal, 14(3), 48- 56.